General Characteristics of Viruses
Viral
structure: Typical viral components are shown in Fig. 2. These
components are a nucleic acid core and a surrounding protein
coat called a capsid. In addition some viruses have a surrounding
lipid bilayer membrane called an envelope.
Fig. 2. The components of helical virus
A. Nucleic acid
• Viral genomes are
either DNA or RNA (not both)
• Nucleic acid may be
single- or double-stranded
Fig. 3. Types
of virus genomes
B. Capsid
• Protein coat
• Protection of Nucleic Acid
• Provides Specificity for Attachment
• Capsomeres are subunits of the
capsid
Fig. 4. Capsid structure
C. Envelope
• Outer
covering of some viruses
• Envelope
is derived from the host cell plasma membrane when the virus buds out
• Some
enveloped viruses have spikes, which are viral glycoproteins that project from
the envelope
• Naked
(non-enveloped) viruses are protected by their capsid alone
Fig. 5. Enveloped
helical virus
2. Size
of viruses:
• Determined by electron microscopy
• Ranges from 20 to 14000 nm in length
Fig. 6. Size
of different viruses
3. Shape
of viruses:
Four basic morphologies
• Icosahedral - efficient
means to conserve and enclose space; form capsomers (planar faces formed by
association of proteins)
• Helical - capsid is
shaped like a hollow protein tube
• Enveloped - outer
covering derived from the host cell's nuclear or plasma membrane and often
possessing spikes or peplomer projections involved in attachment and entry into
a host cell sometimes via their enzymatic activity
• Complex symmetry -
viruses that fit neither of the above categories or which may employ portions
in combination, e.g., bacteriophage
Fig.7. Types
of viral symmetry
4. Host
Range: The specific types of cells a virus can infect in its host
species represent the host range of the virus.
• Animal virus
• Plant virus
• Bacterial virus (bacteriophage)
Host range is determined by attachment sites (receptors)
Important points to remember:
• VIRION
– a complete single viral particle
• Obligatory
intracellular parasites
• Contain DNA or
RNA
• Do not undergo
binary fission
• Sensitive to
interferon
• Contain a protein
coat
• Some are enclosed
by an envelope
• Some viruses have
spikes
• Most viruses
infect only specific types of cells in one host
• Host range is
determined by specific host attachment sites and cellular factors (receptors)
• Viruses replicate
through replication of their nucleic acid and synthesis of the viral protein.
• Viruses do not
multiply in chemically defined media
• All ss-RNA viruses
with negative polarity have the enzyme transcriptase (RNA dependent RNA
polymerase) inside virions.
• Retroviruses and
hepatitis B virus contain the enzyme reverse transcriptase.